Department of Biophysics

PROGRAMMES

P.G. Diploma in Biophysics (One year)
M.Sc. (Biophysics) As per NEP 2020 (Two Year)
M.Sc Biophysics Syllabus As per NEP 2020

COURSES & SYLLABUS

a) M.Sc (Biophysics) by Papers; b) PhD (Biophysics)

1. Post graduate (M.Sc. by Papers, (choice-based credit system)):

The two-year M.Sc. (Biophysics) programme is prescribed according to the choice- based credit system of the University of Mumbai from the academic year 2016-17.

The chief areas covered under the program:

• Molecular and Cellular Biophysics
• Radiation and Medical Biophysics
• Advanced Biophysical Techniques
• Bioinformatics, Molecular Modeling & Drug Design
• Spectroscopy & Bio-crystallography
• Nanobiophysics & Nanomedicine
• Physiological Biophysics
• Environmental biophysics
• rDNA Technology & Protein Engineering
• Biostatistics and Biomathematics

Semester I
Paper code	Paper Nomenclature	Lectures	Credit	Practical/ Laboratory course	Hrs.	Credits	Total Credits
BP-CCT 101	General physico-chemical Principles	60	04	Lab course (BP- LBC) 101	60	02	06
BP-CCT 102	Biomathematics & Biostatistics	60	04	Lab course (BP- LBC) 102	60	02	06
BP-CCT 103	Cellular Biophysics	60	04	Lab course (BP- LBC) 103	60	02	06
BP-CCT 104	Methods in Biophysics	60	04	Lab course (BP- LBC) 104	60	02	06
	Total					24
Semester II
BP-CCT 201	Membrane Biophysics & Ion channels	60	04	Lab course (BP- LBC) 201	60	02	06
BP-CCT 202	Molecular Biophysics	60	04	Lab course (BP- LBC) 202	60	02	06
BP-CCT 203	Biochemistry	60	04	Lab course (BP- LBC) 203	60	02	06
BP-CCT 204	Recombinant DNA Technology & Protein Engineering	60	04	Lab course (BP- LBC)	60	02	06
				204
	Total					24

 

Semester III
Paper code	Paper nomenclature	Lectures	Credit	Practical no. and Code	Hrs.	Credit	Total credits
PB-CCT 301	Physiological Biophysics	60	04	Lab course I (BP-LBC 301)	60	02	06
BP-CCT 302	Bio crystallography & Magnetic Resonance Techniques	60	04	Lab course II (BP-LBC 302)	60	02	06
BP-CCT 303	Radiation Biophysics	60	04	Lab Course III (BP-LBC 303)	60	02	06
PB -CCT 304	Advanced in Biophysical Techniques	60	04	Lab Course IV Research Project /Review literature	60	02	06
	Total						24
Semester IV
BP-CCT 401	Medical Biophysics	60	04	Lab Course I (BP-LBC 401)	60	02	06
BP-CCT 402	Environmental Biophysics	60	04	Lab Course II BP-LBC 402	60	02	06
BP-CCT 403	Nanobiophysics and nanomedicine	60	04	Lab Course III (BP-LBC 403)	60	02	06
BP-CCT 404	Elements of Bioinformatics, molecular modeling and drug design	60	04	Lab Course IV (BP-LBC 404) Research Project	60	02	06
					120		24
	Total						24
	Grand Total (Sem I, II, III & IV)						96
Total credits (M.Sc.) = 96

Detailed syllabus M.Sc I (PDF)

Detailed syllabus M.Sc II (PDF)

PROGRAM OUTCOME

The knowledge of this program will provide an intellectually enlarging experience to students.  The courses attempt to teach critical thinking, problem solving, and communication skills that can be used throughout a life of learning. The program helps to prepare students and make them competitive to enter in the workforce or further their studies in research (Ph.D) at professional colleges, pharmaceutical / biomedical industries, medical schools, Universities  etc.

After completing the M.Sc . in  Biophysics  Program at  University department of Biophysics, the     students will be able to acquire the following skills:

1. Students will demonstrate proficiency in principles of physics, chemistry and mathematical concepts that are needed for a proper understanding of biological process.
2. Students will be able to Integrate knowledge and skills of the physics, chemistry & mathematics with biology and to address research questions.
3. Students will demonstrate knowledge of cell & molecular biology, methods in biophysics, radiation & medical biophysics, physiological biophysics, structure –functions of biological macromolecules, biomathematics and biostatistics, biochemistry & protein engineering, molecular modeling & drug design etc. .
4. Students will show that they have learned laboratory skills, enabling them to purify and characterize biological samples using various spectroscopic and microscopic tools. They will be able to collect, evaluate and interpret scientific data, and employ critical thinking to solve problems in biology  and supporting fields  .
5. Students  will demonstrate  knowledge of various spectroscopy, microscopy and other     analytical tools that are used in biology
6. Students will communicate scientific information in a clear and concise manner both orally and in writing.
7. Student will identify and describe the impact of biophysics  on society.

Program specific outcome  (PSO) :

A Master’s degree of Biophysics will  provides  theoretically and practically  a broad  foundation  in biophysics with particular focus on  cell and membrane biophysics, advanced biophysical techniques, structural biology, radiation and medical biophysics, physiological biophysics, molecular modeling and drug design, environmental biophysics.

In particular, this study includes in depth learning about the structure and function of important biomolecules and cellular systems, biophysical tools as well as physiological biophysics. Students will also learn about methods of protein purification and characterization, measuring the effects of radiation on biological systems, understanding fundamental principles of various biophysical techniques (Fluorescence spectroscopy & Microscopy, UV visible, FTIR, NMR, ESR,  Raman Spectroscopy and other  light scattering) etc. This study will lead further towards problems in medicine, medical physics, use of techniques in radiation for cancer therapy and diagnosis, applications of various spectroscopy tools in biology and medicine.

During the study of this Master’s degree course, the students will also learn fundamental scientific working methods, for example, to work independently on a large-scale project, to design research project, to write scientific contents / research papers and presentation etc.

Course outcome (CO):

Term	Course  code	Outcome
Semester I	BP-CCT101	General Physico-chemical principle: objectives of this course is fundamental understanding of physical, chemical principle which help for quantitative  underrating of biological process. Course  outcomes: By the end of this course the students should be able to develop an understanding of the process of biological system.
	BP-CCT102	Biomathematics & Biostatistics:  Objectives of this course is basic understanding of statistics and mathematics which help the students for quantitative prediction of biological data.  Course outcomes: by  the end of this course students understand basics concepts of statistics  and mathematics  such as mean mode, median, std deviation, types of hypothesis & tests, limits & functions, probability etc.
	BP-CCT103	Cellular Biophysics: objective of this is to understand fundamentals of various types of cells, their structure and function, physical aspects of cells etc. Course outcomes: by the end of this course, the students should able to understand the principles underlining cell function, cell differentiation, cell growth, cell communication etc.
	BP-CCT104	Methods in Biophysics:  objective of this course is to familiarize students with various biophysical methods such electrophoresis, UV visible spectroscopy, fluorescence spectroscopy, circular dichroism, microscopy ( bright field, dark field, fluorescene, SEM, TEM etc). Course outcomes: By the end of the course the student able to understand basics principles of spectroscopic and microscopic techniques and their applications in biological and medical sciences.
	BP-LBC101	Lab course I: objectives of this course is to provide practical training to the students of handling some important equipments, standardization of pH meter, verification of spectroscopic laws and principle etc. Course outcomes: at the end of  the course, the students familiarize with handling of some equipments, understand properties /measurements of biological samples
	BP-LBC102	Lab course II: This course is specially designed specifically statistical analysis of  experimental / biological data. Course outcomes: At the end of this course the student able to statistically evaluate experimental data and its statistical interpretation.
	BP-LBC103	Lab course III: The objectives of this course is to familiarize the students with microscopic techniques, handling of microscope, sample preparation of microscope etc. Course outcomes: by the end of the course the students  should  develop skill of handling of microscopes, study of various biological samples, cell etc
	BP-LBC104	Lab course IV: objectives of the course is to develop a skill of separation of proteins, carbohydrates, lipids using various biophysical tools. Course outcomes: by the end of this course students able to familiarize separation techniques, able to separate and characterize biological samples.
Semester II	BP-CCT201	Membrane biophysics and Ion channels: The objectives of this course is students understand physics of membrane, membrane structure, function,  lipid technology etc. Course outcomes: at the end of the course students should be able to understand various physical and chemical aspects of cell membrane, cell membrane functions etc.
	BP-CCT202	Molecular Biophysics:  The objective of this course is to understand structure and function, stability of biological macromolecules (enzyme, protein, lipids, nucleic acids etc). Course outcomes: by the end of the course student should be able understand the structure  of protein, lipid, carbohydrates. Techniques use to study protein stability, protein folding and significance in biological system.
	BP-CCT203	Biochemistry: This course provides insight of biochemical pathways, signals, cellular level synthesis of biological macromolecules, gene expression in prokaryotes and eukaryotes etc. Course outcomes:  By the end of this course the student able to discuss mechanisms of various pathways takes place in biological system, their impact on biological system, mechanism and regulation of gene expression
	BP-CCT204	rDNA Technology  & Protein Engineering: This course provides students to understand preparation , analysis and manipulation of DNA & RNA, construction of rDNA libraries, mutagenesis, methods of protein sequencing and chemical approach to protein engineering etc. Course outcomes: by the end of this course student able to learn manipulation of genetic materials, construction of recombinant DNA libraries, sequencing strategies of proteins..
	BP-LBC201	Lab course I : the objectives of the course is to provide practical knowledge of physical and chemical aspects of  biological macromolecules (DNA, RNA, Hemoglobin, Sugars).. Course outcomes: by the end of the course students will understand physical and chemical aspects of biological macromolecules, they will learn study of these molecules using spectroscopic techniques.
	BP-LBC202	Lab course II : objectives of the course is to provide hands-on training on estimation of proteins from unknown source, performance of enzyme kinetics experiments etc. Course outcomes:  by the end of the course students will able to isolate and identify the proteins from various sources, student will able to carry enzyme kinetics reaction, they can understand physical and chemical aspects of enzyme reactions.
	BP-LBC203	Lab course III : objectives of the course is to train students for isolation of nucleic acids from various sources, characterize it using various biophysical tools. Course outcomes : at the end of the course students will familiar with isolation of nucleic acid,  restriction endonuclease principle, chromatography techniques ( Gel & paper).
	BP-LBC204	Lab course IV: objective of the course to train  students   for preparation and characterization  of artificial membrane. Experimentally study the physical aspects of  membrane, membrane properties etc. Course outcome: by the end of course students will able to gain practical knowledge of  preparation of artificial membrane, students  will be trained for designing the experiments related to cell membrane.
Semester III	BP-CCT301	Physiological Biophysics: this course will provide insight into functional  aspects of various human organs,  physical and chemical aspects of various organs. Course outcomes : at the end of the course student able to understand basics physiological function of various organs.
	BP-CCT302	Biocrystallography & Magnetic Resonance Techniques: This course will provide basics principals and fundamental understanding of NMR and its various types, ESR, X-Ray diffraction techniques etc. Course outcomes:  by the end of the course students will be familiar with fundamental understanding of magnetic resonance technique and their applications..
	BP-CCT303	Radiation Biophysics : objectives of the course is to understand  the interaction of radiation with matter, living system, biological effects of ionizing and non ionizing radiation etc.  Assessment of radiation effects using various mathematical models. Course outcomes: by the end of course students will gain the basic principle of interaction of radiation with matter and living system, they will be familiar with the knowledge of ionizing radiation and its impact on biological system.
	BP-CCT304	Advanced Biophysical Techniques: Objectives of the course is to make the students familiar with advanced biophysical  techniques such as Differential Scanning colorimetry, Mass spectrometry, force spectroscopy, cryo electron microscopy, patch clamp techniques etc. Course outcomes:  by the end of the course student will gain the knowledge of advanced biophysical techniques, they will understand basics principals and applications in biology and medicine.
	BP-LBC301	Lab course I: the course will provide practical knowledge of analysis of  DNA damage  using physical and chemical methods, protein folding study, ligand binding interaction using SPR, protein structure study using CD ORD, protein-protein interaction using fluorescence spectroscopy, use of fluorescence and differential contrast microscopy for biological samples, protein aggregation etc. Course outcomes: by the end of the course students will gain insight into practical knowledge of DNA damage analysis, protein structure, protein –protein interactions. Students will also become familiar with handling of advance equipments ( fluorescence microscope CD ORD, SPR etc).
	BP-LBC302	Lab course II:  objectives of the course is to provide hands on training of measurement of various surface potentials ( ECG, EEG, ERG, EMG, EOG, etc) , to train students for recording of  surface potential and analysis. Course outcome: by the end of the course student will be able record  and analysis human surface potentials (EEG,ECG, EMG, EOG etc), they will able to interpret inference in normal and abnormal biopotentials.
	BP-LBC303	Lab course III :objectives of the course is to train students with radiation biophysics  experiments. They will train for analysis of radiation effects on biological systems (cells, biological macromolecules), estimation of absorbed dose, study of GM counting system etc. Course outcomes: by the end of the course students will gain knowledge of radiation biophysics, they will able to design  radiation biology experiments. Student will able analysis radiation effects on biological system etc.
	BP-LBC304	Lab course IV: objectives of the course is student understand literature survey on particular areas, material and methodology used in research, how to  write/ interpret  results and discussion, how to design  experiments, how to use various tools for collection of literature,  how organize bibliography etc. Course outcomes:  by the  end of the course students will be able to design of experiments, they will able to analysis of experimental data, they will able to discuss the results obtained on particular problem.
Semester IV	BP-CCT401	Medical Biophysics: This course will provide basics of radiotherapy, nuclear medicine and diagnostics radiology, basics of radiation  protection. Course  outcomes: at the end of the course students will understand fundamental principles of radiation therapy, radio diagnosis , radiation protection and application radiation in medicine. Role of isotopes in medical diagnostics
	BP-CCT402	Environmental Biophysics: Aim of the course is to know the importance of environment, life and environment, Electromagnetic radiation and environment etc. conductance of heart and mass transfer etc. Course outcomes: at the end of course student will able to familiar with environment and its importance to human being.
	BP-CCT403	Nanomedicine and Nanobiophysics: objectives of  the course is to familiar the students with  nanosciences and nanotechnology, understand basics principles of synthesis  of nanostructures, nanotoxicology and application of nanostructures in medicine and biology. Course outcomes : by the end of course students will able to gain knowledge of nanomedicine, nanostructures. Various applications of metallic nanostructures in medicine and biology.
	BP-CCT404	Elements of Bioinformatics, Molecular modeling and drug design:  this course will provide fundamental understanding of bioinformatics, structural bioinformatics, molecular mechanics, denovo ligand and 3D QSAR Course outcomes : by the end of the course students able to gain knowledge of molecular modeling and drug design, they will able to discuss virtual screening etc.
	BP-LBC401	Lab course I: aim of the course is train the students to synthesis and characterize nanostructures, development of nanostructure application in medicine and biology. Course outcomes: by the end of the course students able to synthesis and characterize nanostructures  using various sources (biological, chemical).
	BP-LBC402	Lab course II: aim of the course to expose the students to advanced techniques like Raman Spectroscopy, FTIR, Dynamic Light scattering technique, etc Course outcomes: at the end of course students will able to understand and use of advance techniques for characterization of biological samples.
	BP-LBC403	Lab course III: objectives of the course is develop skill of preparation of bioinformatics database, phylogenic analysis, construction of biomolecule using different tools, analysis of protein-ligand interaction using docking software’s. Course outcomes: by the end of the course students will be to analysis / characterize biomolecuels using various softwares, they will able to predict protein structures, gene annotation etc.
	BP-LBC404	Lab course IV: objectives of the course is to prepare students for research skill, they will learn design of experiments, identification of research problem, how to develop hypothesis, project writing skill, data presentation, writing discussion, bibliography preparation etc. Course outcomes: at the end of course student will be able to design research hypothesis, objectives of research problem, identify suitable material and methods, collect review literature, writing and presentation of research project etc.

 

 

Ph.D. (Biophysics) Programme

The Ph.D. Degree (in Biophysics): involves submission of Dissertation / Thesis to the University based on the research conducted under the supervision of a faculty member (who are recognized teachers of the University for guiding students for Ph. D degree) working at the Department of Biophysics. Students with Master’s Degree (M. Sc) in Sciences (as per the University circular No. 441 of 2005 & VCD’s) are entitled to seek admission for Ph.D. programme of Biophysics.

Program outcome:

The Ph D program in Biophysics has been started in 2007-2008.  The students having post graduate in Biophysics/ Biochemistry/Physics / allied branches of life sciences can enrolled for PhD course.

The PhD program is designed to prepare each student to actively participate in the development and growth in the field of biophysics at all levels in industry / teaching in Universities/ Institutes.  The students research training enhanced by organizing seminars, discussions, invitation  of  world renowned Scientists/ Professors for special lecture, Journal club activity etc. The students will demonstrate depth knowledge  of  various biophysical tools such as  fluorescence spectrophotometer, Raman spectrophotometer, FT-IR, Fluorescence Microscopy, UV Visible spectrophotometer, Fluorescence Microscope, Dynamic Light Scattering, Atomic Force Microscope (Bio AFM), Surface Plasmon Resonance Analyzer, single and 2-D gel electrophoresis. The research projects span across a broad range of topics covering various aspects of structure-function of biological macromolecules; nanostructure-biomolecule interaction; nanostructure based snake venom inhibitor; nanostructure based targated drug delivery system, tumor targeting proteins etc.

ELIGIBILITY & FEES

Eligibility for M.Sc

B.Sc. / B.Sc. (Hons) majoring in Biophysics/Physics/Chemistry/Biochemistry/Microbiology/ Biotechnology/ Forensics science/ Bioinformatics/ any branch of biology with chemistry or physics/ B. Tech (Biotechnology /Bioinformatics). A candidate must have secured minimum 50% marks at B.Sc. examination in the major subject for open candidates. For reserved candidates, University rules and regulation apply.

Fees Structure

The total numbers of seats are 20, out of which seats are allotted to reserve categories as per the rules and regulation of Govt. of Maharashtra and 3% seats are reserved for the students from other universities other than Mumbai University.

Revised Fee structure for M.Sc. courses (wef from the academic year 2009-2010)

Sr. No	Particulars	Amount Rs.
1	Tuition fee	15,000/-
2	Forms and Prospectus	100/-
3	Other fees/ Extracurricular activities	250/-
4	University exam fee	600/-
5	Mark sheet	50/-
6	Laboratory fee	15,000/-
7	Library	1000/-
8	Gymkhana	200/-
9	Admission processing fee	200/-
10	Vice Chancellors fund	20/-
11	Magazine	100/-
12	Identity card	50/-
13	Group Insurance	40/-
14	Student welfare	50/-
15	University sports and Cultural activities	30/-
16	Development fee	500/-
17	Utility	250/-
18	Computer/Internet	500/-
19	e-suvidha	50/-
20	e-charges	20/-
21	Disaster relief fund	10/-
	Total	34,020/-

Note: some fee heads may change time to time

Document verification: 400/- wherever applicable

Refundable deposit Caution Money: 150/-

Library Deposit: 250/-

Laboratory deposit: 400/-

Project fee: 2000/- (wherever applicable)

Registration fee for M.Sc. Part I only: 1000/-

Convocation fee only for MSc. Part II: 250/-

1. NB. Foreign students will have to pay five times of prescribed fees. The fees collected in different heads be spend only for specified purpose for which it has been collected.

Fees are payable by cash in the Allahabad Bank, Kalina Branch, Wadia High School, Ground Floor, Opp. University Campus.

• Tuitions fees once paid are normally not refunded. However, tuition fees will be refunded if the candidates do not attend any theory/practical/seminar class. Application for refund of fees should be addressed to the Registrar, University of Mumbai in duplicate.
• No transference Certificate will be issued to the students who leave the Department, unless they clear all the dues.

Eligibility for PhD:

• Should have M.Sc. degree in Biophysics with minimum 55% marks.
• Candidates qualified in ICMR-JRF/DBT-JRF/CSIR-JRF/ GATE examination under Life Sciences/Biotechnology or PET Biophysics (University of Mumbai) can also apply.

Admission will be subject to their qualifying the departmental interview.
All the applications will be screened and candidates found suitable will be called for interview. Travelling Allowance is not payable by University Department of Biophysics and candidates have to make their own arrangement for stay at Mumbai. Merely fulfilling of the minimum requirements stipulated in the notification would not automatically entitle a person for selection. The decision of the department in this regards shall be final. Applications will be accepted only upon advertisement of PhD notification displayed on Mumbai University website.

STAFF

FACULTY

Name: Dr.Mrs. Varsha Kelkar-Mane (MSc, PhD FRSC, FISC)
Designation: Professor and I/c Head
Area of specialization: Bioprospecting and Nano-Biotechnology
Subjects taught: Biochemistry, Bioprocess technology, Nanotechnology.
Contact details: drvkelkar@mu.ac.in
Profile

 

Dr. Jessy Mariam, PhD
Contact Details: drjessymariam@gmail.com
Designation: Assistant Professor
Subject taught:  Nanobiophysics, Membrane Biophysics, Advanced Biophysical Techniques, Biophysical Chemistry

Dr. Jessy has obtained her Ph.D. in Biophysics from the Department of Biophysics, University of Mumbai. She was awarded the DST-INSPIRE fellowship (2010- 2014) for her doctoral tenure. She obtained her Bachelor’s degree in Microbiology, Biotechnology and a Master’s degree in Biophysics from the University of Mumbai. She worked as a postdoctoral fellow for four years at IIT Bombay. She is an awardee of DBT- Research Associateship, 2015-2018. Her research interests include nanobiophysics, protein-nucleic acid dynamics, and fluorescent probes to study biomolecules. Currently, she is an assistant professor at the Department of Biophysics.

Adjunct faculties:

Prof. B. S. Rao, PhD
Contact Details: bsrao2005@rediffmail.com

Dr. Badanidiyoor Srinivasa Rao joined as an Adjunct Professor in the Department of Biophysics after superannuation from BARC, Mumbai. He started his service as a scientific officer at BARC after graduating from the 12th batch of BARC training school in 1969. He obtained his Ph.D. in biophysics in 1978 from the erstwhile Cancer Research Institute. During his post-doctoral studies (1980-82), he undertook mammalian cell radiobiology in the department of Therapeutic Oncology MCMC, Wisconsin, USA. His research activities have been mainly in the biophysical aspects of radiation quality, radiobiology, radiation cytogenetics, and mutagenesis. He was a recipient of the Madam Marie Curie fellowship of the Europe Commissionerear 1995 and worked on the translocation essay application in bio dosimetry at the University of Leiden, The Netherlands. He has served as an IAEA expert in biodosimetry. He was honored to be on the Editorial Board of the International Journal of Radiation Biology (UK). He also edited the Journal of Radiation Protection and Environment and EMSI newsletter for several years. He retired from BARC in 2005 as the Head of Radiological Physics and Advisory Division.

 

Prof. S. Sivakami, PhD
Contact Details: sivakami_s2000@yahoo.com

Prof. (Mrs.). S. Sivakami joined as an adjunct professor at the Department of Biophysics after superannuation from the Department of Life Sciences, University of Mumbai. She obtained her Ph.D. from the Christian Medical College, Vellore, Tamil Nadu. She had postdoctoral training from the University of Leeds, UK, University of Georgia, USA, and the University of California, Santa Cruz, California, USA. Her main research interests are in the biochemical characterization of enzymes. She has worked on the glycosidases of experimental animals’ brush border membrane of the small intestine and kidney, antioxidant enzymes in experimentally induced diabetes, and non-enzymic glycation in diabetes. More recently, she has studied some enzymes of biotechnological potential from the Cyanobacterium Spirulina platensis. She has been a visiting professor at the National University of Singapore several times. She has published several research papers in reputed national and international journals and authored review articles, popular articles, and a book chapter. She is a fellow of the Maharashtra Academy of Sciences, a fellow of the TWOS, Trieste, Italy, and a member of the editorial board of the Indian Journal of Biochemistry and Biophysics.

Visiting Faculties:

Dr. Sandhya P Kulkarni,
Contact Details: sandhyakulkarni@enablemychild.org

Mrs. Sandhya Kulkarni has been visiting the faculty of the department of Biophysics since 2002. She is a former Assistant Professor of Biophysics, Department of Physiology, BYL Nair Hospital & T N Medical College, and Mumbai. Currently, she is an Associate Director of New Horizons Child Development Centre, Mumbai. Her areas of interest are physiological biophysics, Radiation & Medical biophysics.

 

Former Faculty members:

 

Name: Dr.Prabhakar Dongre
Designation: Professor and Former Head
Areas of research : Radiation biophysics, Structural Biophysics, Nanoscience, Nanotechnology, Molecular dynamics and Bioinformatics
Contact details: drpmdongre@yahoo.co.in

 

 

Dr. Bipin Khade, PhD
Designation: Assistant Professor
Area of specialization: Protein Biophysics, Nano-Biophysics
Subject taught:  Cell & Mole Biophysics, Biochemistry, Membrane biophysics, Advanced Biophysical Techniques, Biophysical Chemistry, Nanotech
Contact Details: drbipinkhade@biophysics.mu.ac.in, bipinbiophysics@yahoo.com

Dr. Bipin S Khade received a Master’s Degree in Biophysics from Govt. Institute of Science Aurangabad, and a Ph.D. in Biophysics from the University of Mumbai. He has qualified for various national Examinations like CSIR-NET JRF, GATE, MH-SET, IISc JRF, etc. He has been awarded the Research Fellowship (2014-2017) from University Grants Commission (UGC), New Delhi, for his Ph.D. studies. He is currently working as Assistant Professor at the Department of Biophysics, University of Mumbai. His research focuses on Nano-Biochemistry, Protein Biochemistry/Biophysics and targeted drug delivery applications. His research interests are finding the structure and functional aspects of various proteins (Serum Albumins, TAU protein, and enzymes) using biophysical approaches. He has published his work in the Journal of Luminescence, Journal of Nanoparticles Research, Cluster Science, and Food Biophysics. He also issued 03 Indian Patents regarding the synthesis of nanostructure and Development of Nano-the KIT for biomedical applications.

RESEARCH FELLOWS

Mr. Vinod Deomani Jaiswal, Ph.D. scholar, JRF-CSIR NET
 Email id: jaisyvinod@outlook.com

 Research interest: My project involves designing nano- based targeted drug delivery systems that could act as a cellular probes for diagnostic and therapeutic purposes. My study involves physical- chemical characterization of these drug delivery systems anda  detailed understanding of the interaction between the drug and the drug delivery systems. In addit , I am ,also probing the structural changes of the protein-based sed carrier systems; stability and kinetics of drug within the conjugates.

 

Mr. Manik N. Waghmare, PhD scholar, JRF-DST PURSE
Email id: waghmare.manik70@gmail.com

Research interest: My research focuses on  understanding the mechanism of interaction and characterization of protein-nanoparticle conjugates (endogenous and exogenous protein) using extensive biophysical techniques. Further, I have defined to study the physico-chemical properties such as biocompatibility, stability, surface activity, partition coefficient, dissociation constant, and in vivo studies. The aforementioned parameters would help to design and develop nanoconjugates for targeted drug delivery application. 

Ms. Dhanashri Pangam, PhD scholar, SARTHI fellow
Email id: pangam2208@gmail.com

Research interest: Being a common threat of rural area, snake envenomation is still one the most neglected human health problems. This problem ensues due to the lack of knowledge of first aid measures, complete reliance on tradition practices and myths, lack of medical emergency facilities, untimely administration of appropriate anti-snake venom (ASV) dose and finally non-specific reactions of ASV. Treatment with Anti snake venom (ASV) is lifesaving, but very risky, because the serum which being used as antivenom is heterologous (produced in different species) and may cause sensitivity reactions in some patients. My project explores the bioactivity of nanomaterials towards venom. Nanomaterials are being used since ancient times in various fields. They also show spectacular interactions with biomaterials. Here we have used silver, gold and zinc nanomaterials against Cobra and Viper venom. Antivenom activity of these nanomaterials is being assayed using in vitro (phospholipase, protease, acidimetry assay and whole blood clotting test) as well as in vivo (chicken egg assay) analysis.

 

Ms. Niyati H. Mudliar,  PhD scholar, GATE Qualified

Email-id: niyatimudliar@gmail.com

Research interest: My project is mainly based on developing sensors for an important biomolecule, Heparin. Apart from its biological significance that is imparted naturally to the blood, Heparin remains the most widely used anticoagulant administered during surgery as prevention towards thrombosis. However, heparin overdose may lead to certain post-surgical complications such as hemorrhages, Heparin-induced thrombocytopenia, etc. which makes it inevitable to monitor the level of Heparin during and after surgery. In my current project, we aim at developing molecular rotor based fluorescent sensors for Heparin, which shall be simple, economic, selective and sensitive, in contrast to the currently practiced indirect methods.

 

ADMINISTRATIVE STAFF

Mrs. Suchita M. Patil
B.Sc. Chemistry
(Lab Assistant)

Miss. Archana Bhilare
(Junior clerk)

Mr. Anil Ghadshi
(Peon)

Neeta Kailas Gaikwad
(Sweeper)

FACILITIES

INFRASTRUCTURE AND LEARNING RESOURCES

• Departmental building

University Department of Biophysics is a single storey building with a grooved semi hexagonal geometry with end to end corridor running throughout the building. At one end of the building Department have HOD office and Seminar/Teaching Hall and on other end one can find M.Sc. Training/Preparation laboratory, Lab 2, Lab 3. Between the extremes ends the middle section of the building has Department Office, Library, Gamma Irradiation laboratory and Lab 1. At the entrance Department has a waiting area facility. Waiting area has a provision of seating arrangement, Departmental Information, Notice Board containing upcoming events and media coverage, etc. Department has given a prime importance to the security affairs keeping entire department under surveillance 24 x 7. Building is totally surrounded by nature which enhances its beauty and thus makes it unique among other department in the University.

• Library

Library is an asset to an institution. Any University is rich because of its rich learning resources. University of Mumbai has a valuable collection of near about 7 lakh books, twenty thousand theses and dissertations, and thousands of periodicals and manuscripts.

In addition to the University Libraries (Rajabai Tower Library and Jawaharlal Nehru Library) Department of Biophysics has its own library which is composed of approximately 400 books including Reference books and Journals. Library also has a valuable set of PhD theses, Project Reports, Dissertations and Published Papers. The collection here is not limited to Biophysical books only. Because of the vastness and the interdisciplinary nature of the subject Department of Biophysics has special collection of Chemistry, Physical Chemistry, Life Sciences, Nano-biophysics, Radiation, Biochemistry, Physiology, Crystallography, Drug delivery, Protein Biophysics, Bioinformatics,Biostatistics, Ecology, Environmental Biophysics, etc. Access is made very feasible just by the recording entry in the library register file. Departmental library extends reading facilities to all post graduate students, PhD students, Post- Doctoral Student, Faculty members and Professors of Biophysics Department.

From the commencement of New Department Building i.e. in last ten years Department has procured books net worth of Rupees 13-14 lakh. Department with all its dedication has positively immobilised the received grant for the library set up. Department of Biophysics is sincerely promoting a high value.

• Teaching Hall

Department has one teaching Hall measuring an area about 700 square feet with a capacity of 60 students. The teaching Hall is utilised for Tutorials, Seminars, Small and Large group teaching, invited talks and Journal Clubs lecture series and conducting Internal examinations. Educational delivery systems such as Over Head Projector (OHP), LCD Projector are effectively used under innovative teaching methodologies in addition to the traditional teaching facilities (white board marker). The teaching Hall is very well equipped with e-learning facilities to be used in the educational delivery systems such as Bio- informatics online and offline software. 24 hours Wi-Fi facilities in the classroom enables students to use their personal laptops for teaching learning purpose. Apart from these facilities the walls of the lecture halls are mounted with the posters of the scope and areas of Biophysics, breakthroughs in the field of Biophysics and Nobel laureates in Biophysics, for motivation for Students. Department has certainly created a positive atmosphere for teaching learning facilities.

• Department Office

An administrative activity is the backbone of any institution and therefore Administrative support is must for smooth functioning of the Department. Day to Day activities of administration involve work related to Record Keeping, billings, budgets, etc. Student enrolment register, Scholarship files, PhD registration files, Purchase files, Budget books, Mark sheet files, Conferences/Seminar record files, Finance and Account files, Repair and Maintenance records and other files have been systematically segregated. With such disciplined procedure it is indeed very helpful to present data to national bodies such as NAAC.

RESEARCH EQUIPMENT FACILITIES

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

RESEARCH

THRUST RESEARCH AREAS

Protein biophysics, Fluorescence Biophysics, Nanobiophysics, Nanomedicine, Molecular Biophysics, Radiation Biophysics

RESEARCH INVESTIGATORS

• Mrs. Varsha Kelkar-Mane Prof &, I/c Head Department of Biophysics
• Bipin S. Khade (Assistant Professor), Department of Biophysics
• Jessy John (Assistant Professor), Department of Biophysics
• B. S. Rao, Adjunct Faculty, Department of Biophysics
• S. Sivakami, Adjunct Faculty, Department of Biophysics
• V. L. Mathe, SPPU, Pune
• Dr. Murli Chilkapati, ACTREC, Navi Mumbai

RESEARCH COLLABORATORS

❖  Advanced Cancer Training and Education Centre, Kharghar, Mumbai

❖  Haffkine Institute of Training and testing, Mumbai

❖  UM DAE Centre for Excellence in Basic Sciences

❖  Smt Savitribai Phule Pune University, Pune

 Projects supported by University of Mumbai 

• Interaction of nanoparticles with model biological system (Protein).
• Image guided intensity modulated radiotherapy: A comprehensive dosimetry and radiobiological study.
• Macromolecular crowding- a biophysical study of implication of volume exclusion effects on bimolecular complexes and them reconstitutes
• Study of diffusion and permeability of nanoparticles through model biological membranes.
• Structure-function properties of nanoparticles bound to biological active molecule in presence of UV radiation.
• In-silico Molecular Docking and Virtual Pharmacokinetic Screening study of Psoraledin and its Derivatives as Phosphatidylinositol3-kinase inhibitors
• Impact of microwave radiation induced heating on vitamins and proteins
• Screening of antithrombotic property of some herbal compounds
• Study of the electrical activity of human brain and heart of the subjects who practice meditation.
• In vitro structure-function based assays of snake venom in presence of nanoparticles

 

Projects sponsored  by Govt of India 

• Oxidative stress of cancer patients undergoing chemotherapy and radiotherapy treatment (BRNS, DAE, Government of India 2009).
• Development of Bioinformatics database for Radiomodifiers and make available through internet to user community (BRNS, DAE, Government of India)
• Design and Development of packaging for Dry Electrodes for Bio-potential measurement and their clinical testing (DRDO, Government of India)

PUBLICATIONS

Research paper publications

Sr No	List of Publications
1.	N Kumari, VL Mathe, CM Krishna, PM Dongre, (2022) BSA-drug-ZnO-PEI conjugates interaction with glycans of gp60 endothelial cell receptor protein for targeted drug delivery: a comprehensive spectroscopic study Journal of Biomolecular Structure and Dynamics, 1-17
2.	BS Khade, P Gawali, M Ali, MN Waghmare, PM Dongre, (2021) Influence of Photon and Electrical Energy in the Nucleation of Silver Nanoparticles Synthesis Journal of Cluster Science, 1-9
3.	BS Khade, PG Gawali, MM Waghmare, PM Dongre, (2021) Adsorption of α-amylase and Starch on Porous Zinc Oxide Nanosheet: Biophysical Study Food Biophysics 16 (2), 280-291
4.	NH Mudliar, PM Dongre, PK Singh, (2021) A Heparin based dual ratiometric sensor for Thrombin International Journal of Biological Macromolecules 167, 1371-1378
5.	NH Mudliar, AM Pettiwala, PM Dongre, PK Singh, (2020) An anionic polyelectrolyte induced aggregate assembly of Thioflavin-T: A prospective platform for Protamine sensing International Journal of Biological Macromolecules 164, 1174-1182
6.	J PENDHARKAR, M WAGHMARE, PG BHATIA, B KHADE, P DONGRE (2021) PHOTO-EXCITATION NATURE OF AROMATIC AMINO ACIDS UNDER ELECTRIC FIELD: A FLUORESCENCE SPECTROSCOPY STUDY Romanian Journal of Biophysics 31 (3)
7.	P PAL, G TALALE, P DONGRE, R SHAH, BIOPHYSICAL TECHNIQUES REVEALED INSIGHT OF POTENTIZED SOLVENT OF ETHANOL-WATER INTERFACE, (2021) Romanian Journal of Biophysics 31 (3)
8.	MN Waghmare, TS Qureshi, CM Krishna, K Pansare, N Gadewal, A Hole, PM Dongre, (2022), β-Lactoglobulin-gold nanoparticles interface and its interaction with some anticancer drugs–an approach for targeted drug delivery
9.	Niyati Mudliyar, Prabhat Singh and PM Dongre, A molecular Rotor Based Dual Ratiometric Sensor, Dyes and Pigments, 2020, Press (DYPI_2020_647)
10.	Vinod J. P M Dongre, Biophysical interaction between silver nanoparticles-albumin interface and curcumin, Journal of Pharmaceutical Analysis, Press, 2020
11.	Manik N. Waghmare  Tazeen S. Qureshi  Afrin N. Shaikh  Dr. Bipin S. Khade  Dr. C. Murali Krishna  Prof. Prabhakar M. Dongre, Functionalized Alpha‐lactalbumin Conjugated with Gold Nanoparticle for Targeted Drug Delivery, ChemistrySelect, 2020, 5:6, 2035-2049
12.	An efficient J-aggregate based fluorescence turn-on and ratiometric sensor for heparin NH Mudliar, PM Dongre, PK Singh, Sensors and Actuators B: Chemical 301, 127089
13.	Lahir, Y. (2019). Graphene and Graphene-Based Nanomaterials Are Suitable Vehicles for Drug Delivery. In Applications of Targeted Nano Drugs and Delivery Systems(pp. 157-189). Elsevier
14.	Albumin nanoparticles conjugates binding with glycan-A strategic approach for targeted drug delivery, N Kumari, VL Mathe, PM Dongre, International journal of biological macromolecules 126, 74-90
15.	Dongre, P. M., & Joshi, A. (2018). A systematic organization of bioinformatics database of radiosensitizers and radioprotectors. Journal of Radiation and Cancer Research, 9(2), 102.
16.	Waghmare, M., Khade, B., Chaudhari, P., & Dongre, P. (2018). Multiple layer formation of bovine serum albumin on silver nanoparticles revealed by dynamic light scattering and spectroscopic techniques. Journal of Nanoparticle Research, 20(7), 185.
17.	Sawant, S., Dongre, H., Ahire, C., Sharma, S., Jamghare, S., Kansara, Y., & Gupta, S. (2018). Alterations in desmosomal adhesion at protein and ultrastructure levels during the sequential progressive grades of human oral tumorigenesis. European journal of oral sciences, 126(4), 251- 262.
18.	Hingane, V. C., Pangam, D., & Dongre, P. M. (2018). Inhibition of crude viper venom action by silver nanoparticles: A biophysical and biochemical study. Biophysics and Physicobiology, 15, 204-213.
19.	Pal, P. D., Dongre, P. M., & Chitre, A. V. (2018). Volume exclusion influences in spectral characteristics of DNA-amino acids complexes. Vibrational Spectroscopy, 99, 137-145.
20.	Y K Lahir, 2018 Review: Influence of nanomaterials on cellular stress and cellular response
21.	Lahir, Y. K. (2018). Impacts of metal and metal oxide nanoparticles on reproductive tissues and spermatogenesis in mammals. Journal of Experimental Zoology, India, 21(2), 593-608.
22.	Khade, B. S., Mathe, V. L., & Dongre, P. M. (2017). α-amylase binding to thermal plasma synthesized zinc oxide nanosheets: A fluorescence study. Journal of Luminescence, 187, 449-456.
23.	Cover image, Volume 23 , Number 8, November 2017, Leading in Oral, Maxillofacial, Head & Neck Medicine
24.	Mariam, J., Sivakami, S., & Dongre, P. M. (2017). Elucidation of structural and functional properties of albumin bound to gold nanoparticles. Journal of Biomolecular Structure and Dynamics, 35(2), 368-379
25.	Sawant, S. S., Dongre, H., Ahire, C., Sharma, S., Kannan, S., Mahadik, S., & Vaidya, M. M. (2017). A nomogram for predicting the risk of neck node metastasis in pathologically node‐negative oral cavity carcinoma. Oral diseases, 23(8), 1087-1098.
26.	Jaiswal, V., Samant, M., Kadir, A., Chaturvedi, K., Nawale, A. B., Mathe, V. L., & Dongre, P. M. (2017). UV Radiation Protection by Thermal Plasma Synthesized Zinc Oxide Nanosheets. Journal of Inorganic and Organometallic Polymers and Materials, 27(5), 1211-1219.
27.	Influence of Meditation on Visual and Auditory Reaction Time in   Young Healthy Volunteers, Indian Journal of Physio Pharmacol, 2017; 61(2) : 100–106
28.	Yogesha, M., Rao, V. G., Martis, E. A., Coutinho, E. C., Gohlke, H., Chidangil, S., & D’Souza, J. S. (2017). Structural features of FAP174, a MYCBP-1 orthologue from Chlamydomonas reinhardtii, revealed by computational and experimental analyses. RSC Advances, 7(81), 51391-51402.
29.	Yogesha, M., Rao, V. G., Martis, E. A., Coutinho, E. C., Gohlke, H., Chidangil, S., & D’Souza, J. S. (2017). Structural features of FAP174, a MYCBP-1 orthologue from Chlamydomonas reinhardtii, revealed by computational and experimental analyses. RSC Advances, 7(81), 51391-51402.
30.	Y K Lahir, 2017 Impacts of Fullerene on Biological Systems, November 2017 DOI: 10.2174/2212707004666171113151624
31.	Impacts of metal and metal oxide nanoparticles on embryos, Citation: Lahir YK. Impacts of Metal and Metal Oxide Nanoparticle on Embryos. Austin Endocrinol Diabetes Case Rep. 2017; 2(1): 1009.Austin Endocrinol Diabetes Case Rep – Volume 2 Issue 1 – 2017Submit your Manuscript | www.austinpublishinggroup.com Lahir. © All rights are reservedAustin Endocrinology and Diabetes Case Reports
32.	Impacts of metal and metal oxide nanoparticles on embryos. Lahir YK. Impacts of Metal and Metal Oxide Nanoparticle on Embryos. Austin Endocrinol Diabetes, 2017; 2(1): 1009.
33.	Guru, A. D., Lahir, Y. K., & Mohite, V. T. (2017). Study on some biochemical aspects of alimentary canal of millipedes Harpaphe haydeniana (Wood). Journal of Experimental Zoology, India, 20(2), 789-794.
34.	Mariam, J., Sivakami, S., & Dongre, P. M. (2016). Albumin corona on nanoparticles–a strategic approach in drug delivery. Drug delivery, 23(8), 2668-2676.
35.	Pal, P. D., Dongre, P. M., & Chitre, A. V. (2016). Implications of Volume Exclusion: A Look at Thermodynamic Perspective of DNA-Hemoglobin Complexes and Their Reconstitutes Under Macromolecular Crowding. Journal of fluorescence, 26(1), 355-362.
36.	Y K Lahir, ROLE OF NANOMATERIALS IN THE DEVELOPMENT OF BIOSENSORS, G.J.B.B., VOL.5(2)2016: 146-163
37.	Gurve, D., Muthurajan, H., Karnik, P., Deshpande, A., Srivastava, A. K., Mukhopadhyay, K., & Dongre, P. M. (2016, March). Novel algorithm for coherence level measurement using RR interval of ECG signal. In 2016 International Conference on Wireless Communications, Signal Processing and Networking (WiSPNET) (pp. 2187-2191). IEEE.
38.	Rao, V. G., Sarafdar, R. B., Chowdhury, T. S., Sivadas, P., Yang, P., Dongre, P. M., & D’Souza, J. S. (2016). Myc-binding protein orthologue interacts with AKAP240 in the central pair apparatus of the Chlamydomonas flagella. BMC cell biology, 17(1), 24.
39.	Tari, D., Haryan, S., Patankar, K., Jaiswal, V., Samant, M., Sivakami, S., & Dongre, P. M. (2016). A simple egg membrane model for understanding diffusion characteristics of nanoparticles and amino acids. Available at SSRN 2783837
40.	Y K Lahir, Application of time and length scale concept in biological systems, Biochem. Cell. Arch.Vol. 16, No. 2, pp. 231-237, 2016
41.	Lahir, Y. H. (2016). Understanding the basic role of glycocalyx during cancer. Journal of Radiation and Cancer Research, 7(3), 79.
42.	Lahir Y.K. Some Aspects of Interactions between Nanomaterials and the Cytoskeleton of Eukaryotic cells, January 2016, DOI: 10.23880/ACT-16000111
43.	Y K Some Biophysical And Biochemcial Interactions Of Nanomaterials On Protein Oxidation In Biological System Report and Opinion 2016;8(7)
44.	Shirsekar, P. P., Kanhe, N., Mathe, V. L., Lahir, Y. K., & Dongre, P. M. (2015). Interaction of zinc oxide Nanoparticles with human red blood cells. Bionano frontier
45.	Lahir, Y. K. (2015). Lipid oxidation in biological systems: Biochemical, Biological and Biophysical aspects. Global JB B, 4, 224-233.
46.	Kumar, L. Y. (2015). Role and adverse effects of nanomaterials in food technology. Journal of toxicology and health, 2(1), 2.
47.	Lahir, Y. K. (2015). A dynamic component of tissues-Extracellular matrix: Structural, functional and adaptive approach. Biochem Cell Arch, 15, 331-47.
48.	Lahir, Y. K. (2015). Lipid oxidation in biological systems: Biochemical, Biological and Biophysical aspects. Global JB B, 4, 224-233.
49.	Kumar, L. Y. (2015). Role and adverse effects of nanomaterials in food technology. Journal of toxicology and health, 2(1), 2.
50.	Mariam, J., Sivakami, S., Kothari, D. C., & Dongre, P. M. (2014). Bioactivity of albumins bound to silver nanoparticles. The protein journal, 33(3), 258-266.
51.	Pant, M. P., Mariam, J., Joshi, A., & Dongre, P. M. (2014). UV radiation sensitivity of bovine serum albumin bound to silver nanoparticles. Journal of Radiation Research and Applied Sciences, 7(4), 399-405.
52.	Pardeshi, P., Nawale, A. B., Mathe, V. L., Lahir, Y. K., & Dongre, P. M. (2014). Effects of zinc oxide nanoparticles on the hepatic tissue of chicken embryo: a histopathological approach. Bio Nano Front, 2, 176-180.
53.	Pal, P. D., Dongre, P. M., & Chitre, A. V. (2014). “Is Macromolecular Crowding Overlooked?”- Effects of Volume Exclusion on DNA-Amino Acids Complexes and Their Reconstitutes. Journal of fluorescence, 24(4), 1275-1284.
54.	Bhogale, A., Patel, N., Mariam, J., Dongre, P. M., Miotello, A., & Kothari, D. C. (2014). Comprehensive studies on the interaction of copper nanoparticles with bovine serum albumin using various spectroscopies. Colloids and Surfaces B: Biointerfaces, 113, 276-284.
55.	Lahir, Y. K. (2014). Morphological aspects of intercellular communication. Bionano Frontier, 7(1), 03-17.
56.	Lahir Y K, Morphological aspects of inter cellular communication, Bionano Frontier, 2014, 7(1): 03-11
57.	Bhogale, A., Patel, N., Mariam, J., Dongre, P. M., Miotello, A., & Kothari, D. C. (2013, February). Study of interaction of ZnO nanoparticles with human serum albumin using fluorescence spectroscopy. In AIP Conference Proceedings (Vol. 1512, No. 1, pp. 130-131). AIP.
58.	Bhogale, A., Patel, N., Sarpotdar, P., Mariam, J., Dongre, P. M., Miotello, A., & Kothari, D. C. (2013). Systematic investigation on the interaction of bovine serum albumin with ZnO nanoparticles using fluorescence spectroscopy. Colloids and Surfaces B: Biointerfaces, 102, 257-264.
59.	Dr P M Dongre, Effect on cognitive function from mobile phone use, Bionano frontier, Vol. 5. (1),2012
60.	Dr P M Dongre et al, Impact of microwave radiation heating on vitamins and proteins, Bionano Frontier, Vol. 5. (1),2012
61.	Sharma, S. D., Dongre, P., Mhatre, V., & Heigrujam, M. (2012). Evaluation of automated image registration algorithm for image-guided radiotherapy (IGRT). Australasian physical & engineering sciences in medicine, 35(3), 311-319
62.	Shelke, J., Meshram, G., & Dongre, P. (2012). SYNTHESIS OF 2-OXOQUINOLINE-3-CARBOXAMIDE OF AMPICILLIN AND AMOXICILLIN AS INHIBITORS OF PENICILLIN BINDING PROTEIN 1A OF PSEUDOMONAS AERUGINOSA. Acta poloniae pharmaceutica, 69(4), 623-628.
63.	Mariam, J., Dongre, P. M., & Kothari, D. C. (2011). Study of interaction of silver nanoparticles with bovine serum albumin using fluorescence spectroscopy. Journal of fluorescence, 21(6), 2193.
64.	Sharma, D. S., Dongre, P. M., Mhatre, V., & Heigrujam, M. (2011). Physical and dosimetric characteristic of high‐definition multileaf collimator (HDMLC) for SRS and IMRT. Journal of applied clinical medical physics, 12(3), 142-160.
65.	Yadav, A. A., Barote, M. A., Dongre, P. M., & Masumdar, E. U. (2010). Studies on growth and characterization of CdS1− xSex (0.0≤ x≤ 1.0) alloy thin films by spray pyrolysis. Journal of Alloys and Compounds, 493(1-2), 179-185.
66.	Meshram, G., Shelke, J., & Dongre, P. (2010). Synthesis, antibacterial study and molecular docking of some acrylic amide derivatives of ampicillin and amoxicillin. J Pharm Res, 3(10), 2371-2374.
67.	Khurana, R. R., Patel, V. V., Dongre, P. M., & Nagare, B. J. (2009) In-silico Molecular Docking and Virtual Pharmacokinetic Screening study of Psoralidin and its Derivatives as Phosphatidylinositol 3-Kinase Inhibitors. Trends in Bioinformatics 5(1 &2):163-178
68.	Bansode, T. N., Dongre, P. M., & Dongre, V. G. (2009). Synthesis, antibacterial and antifungal activity of 1, 3-di (2-substituted 10H-phenothiazin-10-yl) propan-1-one. Pharmaceutical chemistry journal, 43(6), 311.
69.	Simple, Efficient Synthesis, Antibacterial activity and Molecular Docking Study of 3- (1Hbenzimidazol- 2-yl)-2-chloroquinolines Compounds
70.	Shilpa Patil, P M Dongre , Silver Nano Particles Modified Chitosan Beads: An Improved Stability of Cellulase by Immobilization
71.	Y K Lahir, Advances in Clinical Toxicology Some Aspects of Interactions between Nanomaterials and the Cytoskeleton of Eukaryotic cells Some Aspects ofInteractions between Nanomaterials and the Cytoskeleton of Eukaryotic cells Advances in Clinical Toxicology, Advances in Clinical Toxicology, volume 1 issue 2

Book Chapters

Sr No                                                                                  Book Chapters

Dr Y K Lahir, Graphene 1           APPLICATIONS OF TARGETED NANODRUGS AND DELIVERY SYSTEMS Publisher: Elsevier Inc, October 2018

Dr P M Dongre, Title of Chapter: Gold Nanoparticle–Assisted Radiation Therapy 2           Title of Book: Radiation in Medicine and Biology, Edited by Pandit B. Vidyasagar, Sagar S.Jagtap, and Omprakash Yemul, Copyright © 2016 Pan Stanford Publishing Pte. Ltd.ISBN 978-981-4745-92-5(Hardcover), 978-981-4745-93-2 (eBook) www.panstanford.com

INNOVATION & AWARDS 

Research, Innovation and Extension 

Digitization of bioinformatics database of radiosensitizers and radioprotectors 

Radiotherapy is regarded as one of the most important therapeutic modalities for the treatment of malignant lesions. This field is undergoing rapid advancements in the recent times. With the use of radio-sensitizing and radioprotective agents, the course of radiotherapy  has improved the sensitization of tumour cells and protection of normal cells, respectively

Radiosensitizers makes tumour cell more sensitive to radiation therapy which increase the effectiveness of cancer treatment where radioprotectors are the compounds that reduce the damage/ spare normal tissue. Several of these compounds have been studied using appropriate biological model system and their efficacy is reported in literature. The literature of these compounds is highly scattered and it is essential to have all this information on a single platform which will further help in the improvement in radiotherapy treatment.

Hence, department has developed bioinformatics database of radiosensitizers and radioprotectors using information available in PubMed, scientific journals and other scientific sources. The collected information of these compounds is systematically organized on a single platform where user can browse typical information of the compound pertaining to mainly structural features, radiobiological aspects, biological targets, clinical trials, pharmacological aspects, toxicity etc. The direct link of these compounds is available on University of Mumbai web portal (www.mu.ac.in) / http://bioph.mu.ac.in/Welcome/ as well as Department of Biophysics web portal. This comprehensive database will be useful for clinicians, researchers and radiation workers etc. Along with this exhaustive exercise, we have invited feedback from users for further improvement or revision of this bioinformatics database. Similarly interested researcher, clinician can be uploaded his/her experimental data (peer reviewed).

Finance Support: Board of Research in Nuclear Sciences, Department of Atomic Energy, Govt of India

Nanostructure-based snake venom inhibitor 

Snake bite is one of the most important public health problems worldwide, specifically in tropical countries. It is a common occupational hazard mainly in rural areas. There is significant morbidity and mortality reported worldwide. In India near about 52 thousand morbidity reported per year. At present there is no reliable treatment established due various physiological/ biochemical problems.

Snake venom is a complex mixture of various biologically, and pharmacologically active  proteins and polypeptides. The current treatment available for snake bite  is intravenous administration of anti-snake venom (ASV) which neutralizes snake venom. ASV is the polyvalent antibodies manufactured by using surrogate animals   (e.g. Horse).  These animals are immunized with the snake venom and then antibodies are collected from blood and purified. Treatment with Anti snake venom (ASV) is lifesaving but very risky and may not be safe sometimes, and may cause sensitivity reactions in patients (chills,   nausea and fever, hypersensitivity or anaphylactic shock). Hence there is a need to focus on alternative treatment considering the limitation of available treatment. In this research work, Silver/ gold nanostructures were used as potential inhibitors against snake venom. We have shown that the action of venom is reduced up to 95% using these nanostructures.

Teaching, Training kit for nanostructure preparation 

To train the students (high school, graduate, post graduate, research scholar etc) in the field of nanoscience and nanotechnology, we have developed teaching, training kit for synthesis of nanostructures (gold and silver). The kit provides desired chemicals/ constitutes, from which the students can easily prepare these nanostructures by following the given simple protocol. A major advantage of this kit eliminates sophisticated equipment’s and other expenditures.

Key features:

1. First time will be introduced in the educational system
2. Simple, effective & economical kit for training for the understanding synthesis of nanostructures
3. Minimum requirement, eco-friendly, customized, and time-saving
4. Useful to high school, Jr & Sr College students

 

 

Crystal for cell / biomolecules storage

Storage/preservation of cells is extremely important to ensure that quality is maintained before usage of cells. Several industries such as the food, pharmaceutical, and horticultural industries require extensive use of various types of cells. Hence there is a requirement to preserve cells so that they can be used either directly or for further research at the appropriate time. In order to preserve these cells, expertise from a variety of disciplines including but not limited to engineering, biology, biotechnology, cryobiology etc is required to design protocols that enable the development of precise and reliable preservation methods. There are many methods that are used today to preserve cells, such as cryopreservation, hypothermic preservation, vitrification, freeze drying etc. All these methods have advantages, disadvantages, and several limitations.

Our research team has developed a simple and innovative technique where cell / biological materials (DNA) can be preserved for a longer time. Its unique crystal can preserve cells longer time without providing nutrients, it’s cost-effective, novel, and simple. Not much expertise is required. Testing of this technique, experimentation, and data collection/validation is in progress.

Teaching training membrane model for understanding diffusion characteristics in biology 

Diffusion is an important phenomenon that occurs in a living system for carrying out various biological activities. This has been quantitatively described using mathematical models and proved experimentally. The outcome of these studies has been used for designing the experiments in vivo and vitro .The simplest mechanism by which molecules can across the membrane is passive diffusion. During passive diffusion, a molecule simply crosses the membrane and enters the aqueous solution at the other side of the membrane.

We have developed a simple biological membrane model for understanding diffusion characteristics in biology. The chicken eggshell has been used and prepared as a membrane model to perform passive diffusion. It diffusion has been performed for Silver Nanoparticles and amino acids against gravity, towards gravity, and lateral state. This membrane has been systematically characterized with the help of X-ray Scattering and Scanning Electron Microscopy. This model is being established at a large-scale level.

Teaching, Training kit for nanostructure preparation 

To train the students (high school, graduate, postgraduate, research scholar etc) in the field of nanoscience and nanotechnology, we have developed a teaching and training kit for the synthesis of nanostructures (gold and silver). The kit provides desired chemicals/ constitutes, from which the students can easily prepare these nanostructures by following the given simple protocol. A major advantage of this kit is that it eliminates sophisticated equipment and other expenditures.

Key features:

1. First time will be introduced in the educational system
2. Simple, effective & economical kit for training for the understanding synthesis of nanostructures
3. Minimum requirement, eco-friendly, customized, and time-saving

Useful to high school, Jr & Sr College students

ACHIEVEMENTS/AWARDS

M.Sc. and Ph.D. Students 

Ph.D. Awarded

Sr No	Student Name	Topic Name	Fellowship awarded	Degree awarded Year	Degree awarded in
1	Ms. Jessy John	A study of interaction of Nanoparticles with model Biological Systems using Biophysical Approaches.	DST-INSPIRE	2014	Biophysics
2	Mr. Dayanand Sharma	Image guided intensity modulated radiotherapy: A comprehensive dosimetry and radiobiological study.		2014	Biophysics
3	Priyanka Pal	Macromolecular crowding- a biophysical study of implication of volume exclusion effects on bimolecular complexes and their reconstitutes	DST-INSPIRE	2016	Biophysics
4	M.Venkatramanan	Characterization of Flagellar associated protein 174 (FAP174) from the green chlorophyte Chlamydomonas reinhardtii	DBT-SRF	2016	Biophysics
5	Mr. Bipin S Khade	Investigation of structure function relationship of model protein in presence of metallic nanoparticle	UGC-RGNF	Feb-2018	Biophysics
6.	Mrs. Aditi Lohot	A systematic study of the electrical activity of human brain and heart of the subjects who practice meditation.	Non-Net	May 2018	Biophysics
7.	Mrs. Vrushali Hingane	In vitro structure-function based assays of snake venom in presence of nanoparticles	Non-Net	May 2018	Biophysics
8.	Mrs. Sharda Sawant	A study of desmosomal and hemi-desmosomal adhesion junctions in neoplastic progression of human oral cancer using transmission electron microscopy.	————	2019	Biophysics

Achievements

1. Venkatramanan G. Rao (PhD Scholar), DBT- Senior Research Fellow
2. Jessy Mariam, ( PhD scholar, 2008-14), DST INSPIRE fellowship, New Delhi
3. Bipin S Khade (PhD student), RGNF. UGC XII plan
4. Priyanka Pal, (PhD student) DST INSPIRE fellowship. New Delhi
5. Mugdha Dhurandhar (MSc Student 2015-17), DST-INSPIRE, New Delhi, India
6. Vrushali Khedekar ( MSc Student 2015-17), DST-INSPIRE, New Delhi, India
7. Zehra Haider (MSc Student, 15-17), Scholarship through NGO
8. Jessy Mariam a Ph. D scholar, Second prize for best research paper presentation in International Conference on Nanobiotechnology: An Interface between Physics and Biology, Jamia Millia Central University, New Delhi, on Dec 2-4, 2010.
9. Bipin Khade, MH-SET (2015), CSIR-NET (Dec 2015 (AIR 66), June 2016 (AIR 141)
10. Bipin Khade, D scholar, Best research paper presentation award, IISER Mohali, March 2017
11. Best paper presentation award Metalions, NEERI.
12. Suraj Singh, Silver medal Avishkar 2017
13. Manik Waghmare, Silver medal at Avishkar 2017-18 final University Round
14. Vinod Jaiswal, Best research paper award- “International Conference on Nanotechnology for Human Welfare”.
15. Vinod Jaiswal- NET  (CSIR-UGC 2018) Qualified
16. Prasad Kapkar-NET (CSIR-UGC 2018) qualified
17. Prasad Kapkar MH-SET (2018) qualified
18. Dhanashri Pangam, Silver medal at Avishkar 2018-19 final University Round
19. Dhanashri Pangam, PhD scholar, SARTHI fellow, 2019
20. Vinod Jaiswal, University Gold medal-Avishkar 2019-2020
21. Vinod Jaiswal, Innovation award by Nehru Science Centre, 2019-2020
22. Vinod Jaiswal, Inter-University Gold medal-Avishkar 2019-2020
23. Tazeen Qureshi Sc Student DST-INSPIRE award 2020
24. Puja Shah, M.Sc Student DST-INSPIRE award 2021

ORGANIZED CONFERENCES/SYMPOSIA.

Sr. No	Year	Title of organized conference/symposia
1.	2022	Outreach program for high school students, March, 2022
2.	2021	Outreach program for high school students, 18,19 Feb
3.	2021	Three Days Mini Symposium – Workshop on Biophysics
4.	2020	Carrier Guidance-Biophysics-September 2020
5.	2019	Workshop On Exploring Biophysical Techniques For Protein Purification And Characterization, Nov, 2019
6.	2019	Two Days Mini Symposium – Workshop on Biophysics, August 2019
7.	2018	Two Days Mini Symposium – Workshop on Biophysics, august 2018
8.	2016	International Conference on Metal Ions in Biology, November 28-30
9.	2016	National Seminar on “Impact of Climate change on Human health with respect to vector borne and water borne diseases” in association with CSIR NEERI, Mumbai, June 2016
10.	2016	Biophysics Week – “Workshop and Seminar on advanced Biophysical techniques”, March, 2016
11.	2015	National Seminar-Emerging Opportunities and Rewarding Carrier of Radiation and Cancer Research (CAREER-RCR), July, 2015
12.	2014	Workshop on Biopotential and Signal Processing, September, 2014
13.	2014	Workshop on Advances in Bioimaging, Feb, 2014
14.	2013	National Symposium (IBS) on frontiers of Biophysics, Biotechnology, Bioinformatics, Jan, 2013
15.	2012	National Seminar on “Structural biology and bioinformatics”, Feb, 2012
16.	2011	National Seminar on-Advanced techniques in Radiation Biophysics, Feb, 2011